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fluorescein avidin direct conjugate system  (Vector Laboratories)


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    Structured Review

    Vector Laboratories fluorescein avidin direct conjugate system
    Fluorescein Avidin Direct Conjugate System, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 414 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescein+avidin+direct+conjugate+system/Fluorescein+Avidin+DCS%2C+Cell+Sorting+Grade/pmc11524632-35-6-13
    Average 95 stars, based on 414 article reviews
    fluorescein avidin direct conjugate system - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: Topical Platelet Exosomes Reduce Senescence Signaling in Human Skin: An Exploratory Prospective Trial
    Article Snippet: Samples were incubated with rabbit monoclonal anti-γH2AX (1:400, 9,718; Cell Signaling), followed by a goat antirabbit biotinylated secondary antibody (1:200, PK-6101; Vector Labs, Burlingame, CA). .. After washing, samples were incubated with fluorescein avidin direct conjugate system (1:500, A-2011; Vector Labs), washed, and then cross-linked in 4% paraformaldehyde in phosphate-buffered saline. .. After washing and dehydration in graded cold ethanol solutions, tissues were air-dried, then denatured in hybridization mix (70% deionized formamide [Sigma], 25 mM MgCl2, 1 M Tris pH 7.2, 5% blocking reagent [Roche] containing 2.5 μg/mL Cy‐3‐labelled telomere‐specific peptide nucleic acid probe [PANAGENE]), and then incubated in a dark, humidified chamber for hybridization.

    Avidin-Biotin Assay:

    Article Title: Topical Platelet Exosomes Reduce Senescence Signaling in Human Skin: An Exploratory Prospective Trial
    Article Snippet: Samples were incubated with rabbit monoclonal anti-γH2AX (1:400, 9,718; Cell Signaling), followed by a goat antirabbit biotinylated secondary antibody (1:200, PK-6101; Vector Labs, Burlingame, CA). .. After washing, samples were incubated with fluorescein avidin direct conjugate system (1:500, A-2011; Vector Labs), washed, and then cross-linked in 4% paraformaldehyde in phosphate-buffered saline. .. After washing and dehydration in graded cold ethanol solutions, tissues were air-dried, then denatured in hybridization mix (70% deionized formamide [Sigma], 25 mM MgCl2, 1 M Tris pH 7.2, 5% blocking reagent [Roche] containing 2.5 μg/mL Cy‐3‐labelled telomere‐specific peptide nucleic acid probe [PANAGENE]), and then incubated in a dark, humidified chamber for hybridization.

    Saline:

    Article Title: Topical Platelet Exosomes Reduce Senescence Signaling in Human Skin: An Exploratory Prospective Trial
    Article Snippet: Samples were incubated with rabbit monoclonal anti-γH2AX (1:400, 9,718; Cell Signaling), followed by a goat antirabbit biotinylated secondary antibody (1:200, PK-6101; Vector Labs, Burlingame, CA). .. After washing, samples were incubated with fluorescein avidin direct conjugate system (1:500, A-2011; Vector Labs), washed, and then cross-linked in 4% paraformaldehyde in phosphate-buffered saline. .. After washing and dehydration in graded cold ethanol solutions, tissues were air-dried, then denatured in hybridization mix (70% deionized formamide [Sigma], 25 mM MgCl2, 1 M Tris pH 7.2, 5% blocking reagent [Roche] containing 2.5 μg/mL Cy‐3‐labelled telomere‐specific peptide nucleic acid probe [PANAGENE]), and then incubated in a dark, humidified chamber for hybridization.



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    95
    Vector Laboratories fluorescein avidin direct conjugate system
    Fluorescein Avidin Direct Conjugate System, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescein+avidin+direct+conjugate+system/Fluorescein+Avidin+DCS%2C+Cell+Sorting+Grade/pmc11524632-35-6-13
    Average 95 stars, based on 1 article reviews
    fluorescein avidin direct conjugate system - by Bioz Stars, 2026-09
    95/100 stars
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